Simplicillium lepidopterorum

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Taxonavigation

Ordo: Hypocreales
Familia: Cordycipitaceae
Genus: Simplicillium

Name

Simplicillium lepidopterorum W.H. Chen, C. Liu, Y.F. Han, J.D. Liang & Z.Q. Liang sp. nov. – Wikispecies link – Pensoft Profile

Etymology

The epithet lepidopterorum refers to an insect host in order Lepidoptera.

Diagnosis

Characterized by phialides always being solitary and rather long and narrow, 15.3–26.2 × 0.7–1.4 μm, Conidia adhering in globose slimy heads, mostly ellipsoidal, 1.6–2.4 × 1.4–1.7 μm. Octahedral crystals absent. The reverse of colony was pale white.

Type

CHINA, Guizhou Province, Huaxi District (26°23'25.92"N, 106°41'3.35"E), 31 July 2018, Wanhao Chen, holotypeGZAC GY2913, ex-type culture GZAC GY29131, sequences from isolated strain GY29131 has been deposited in GenBank with accession numbers: ITS = MN006246, LSU = MN006251, RPB1 = MN022273 and TEF = MN022265.

Description

Colonies reaching 48–51 mm in diameter in 14 d on PDA; white; reverse pale white. Hyphae septate, hyaline, smooth-walled, 1.1–2.2 μm wide. Phialides arising from aerial hyphae, gradually tapering towards the apex, without basal septa, always solitary and rather long and narrow, 15.3–26.2 × 0.7–1.4 μm. Conidia adhering in globose slimy heads, ellipsoidal to fusiform, hyaline, smooth-walled, 1.6–2.4 × 1.4–1.7 μm. Octahedral crystals absent.

Host

Carpenter worm (Lepidoptera)

Distribution

Huaxi District, Guizhou Province, China

Remarks

Simplicillium lepidopterorum was easily identified as belonging to Simplicillium because of its solitary phialides, conidia adhering in globose slimy heads, and lack of octahedral crystals. Comparing with the typical characteristics of 12 species (Table 2), S. lepidopterorum could easily distinguished from other species by having the phialides always solitary and rather long and narrow, 15.3–26.2 × 0.7–1.4 μm. Conidia ellipsoidal (1.6–2.4 × 1.4–1.7 μm), adhering in globose slimy heads, and in having the octahedral crystals absent. Based on ITS and LSU rDNA, S. lepidopterorum is phylogenetically close to S. cicadellidae and S. formicidae. However, S. lepidopterorum has ellipsoidal conidia, longer phialides (15.3–26.2 × 0.7–1.4 μm), and the reverse of colony was pale white.

Key

Key — W. Chen , C. Liu , Y. Han , J. Liang , W. Tian , Z. Liang

1		Conidia in globose or subglobose heads    2
–		Conidia in chains or solitary    11
2		Macro- and microconidia present    3
–		Only one type of conidia present    4
3		Octahedral crystals present     S. lamellicola
–		Octahedral crystals absent     S. coffeanum
4		Octahedral crystals present    5
–		Octahedral crystals absent     9
5		Conidia cylindrical     6
–		Conidia subglobose or ellipsoidal     7
6		Chlamydospores present, conidia 4.2–6.5 × 1.2–2.0 μm     S. aogashimaense
–		Chlamydospores absent, conidia 3.0–4.5 (–5.0) × 1.0–2.0 μm     S. cylindrosporum
7		Conidia subglobose to ellipsoidal    8
–		Conidia oval or ellipsoidal to subcylindrical, 1.5–3.0 × 0.7–1.3 μm     S. lanosoniveum
8		Conidia subglobose to ellipsoidal, 2.3–4.0 (–4.5) × 1.5–3.3 μm     S. subtropicum
–		Conidia globose to subglobose, sometimes ellipsoidal, 2.5–3.5 × 1.8–2.5 (–2.8) μm     S. minatense
9		Conidia ellipsoidal    10
–		Conidia filiform to fusoid     S. formicidae
10		The reverse of colony pale white, phialide 12.9–18.3 × 0.8–1.1 μm     S. cicadellidae
–		The reverse of colony yellowish, phialide 15.3–26.2 × 0.7–1.4 μm     S. lepidopterorum
11		Denticles present in conidiogenous cell (phialide)     S. sympodiophorum
–		Denticles absent in conidiogenous cell (phialide)    12
12		Macro- and microconidia present     S. calcicola
–		Only one type of conidia present    13
13		Conidia ellipsoidal    14
–		Conidia fusoid to filiform, form zigzag chains     S. filiforme
14		Conidia in branched or unbranched chains, 3.5–5.0 × 1.0–1.5 μm     S. chinense
–		Conidia in short imbricate chains, 2.5–3.5 × 1.0–2.0 μm     S. obclavatum

Original Description

• Chen, W; Liu, C; Han, Y; Liang, J; Tian, W; Liang, Z; 2019: Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China MycoKeys, 58: 83-102. doi

Images

Figure 5. Simplicillium formicidaeA isolated substrate an infected ant (Hymenoptera) B–C culture plate, showing the front (B) and the reverse (C) of the colony, cultured on PDA medium D, E phialides solitary, conidia adhering globose slimy head and conidia F conidia. Scale bars: 10 mm (B, C), 10μm (D, E, F).