ITS PIPE version 1.0 UNITE database version v6.dynamic Allowed mismatches on the barcode 1 Allowed mismatches on the primer 6 Minimum read length 300 Minimum read quality average (Phred score) 20 Maximum ambiguities in the sequence allowed 0 Maximum homopolymer length allowed 8 Minimum number of raw reads per sample 2000 Minimum number of good quality reads per sample 1500 Samples reads count normalisation yes Clustering similarity threshold (%) 97 Minimum number of reads for selecting dominant OTUs 4 Trimming length 360 Trimming quality average 30